Multi-Target Analysis and Suspect Screening of Xenobiotics in Milk by UHPLC-HRMS/MS

The development of suspect or non-target screening methods to detect xenobiotics in biological fluids is essential properly understand the exposome and assess its adverse health effects on humans. In order fulfil that aim, biomonitorization human compulsory. However, these are not yet extensively developed, especially for polar organic biofluids such as milk, most works only focused certain analytes interest. this work, a multi-target analysis method determine 245 diverse milk by means Ultra High Performance Liquid Chromatography (UHPLC)-qOrbitrap was developed. Under optimal conditions, liquid samples were extracted with acetonitrile presence anhydrous Na2SO4 NaCl, extracts cleaned-up protein precipitation at low temperature Captiva Non-Drip (ND)—Lipids filters. optimized validated two concentration-levels (10 ng/g 40 ng/g) obtaining satisfactory figures merit more than 200 compounds. applied several samples, including commercial breast provided 4 healthy volunteers. Moreover, extended perform 17,000 xenobiotics. All all, detected, highlighting food additives (benzothiazole) phytoestrogens (genistein genistin) stimulants (caffeine), plasticizers (phthalates), UV filters (benzophenone), pharmaceuticals (orlistat) samples.